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ATCC79878 pCMV5.32A7
基本信息
资源编号 ATCC79878
资源名称 pCMV5.32A7
种属 Homo sapiens, human
安全等级 1
应用领域 in another host, produces protein coagulation factor VII (serum prothrombin conversion accelerator)
培养方法
培养基 ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
详细说明
描述 Restriction digests of the clone give the following sizes (kb): BamHI--4.4, 3.0; BstEII--7.6; SstI--7.0, 0.5; NotI--7.6; EcoRI--5.0, 2.8.
Digestion with EcoRI produces fragments of 5.0 and 2.4 kb.
Bacteria may grow slowly due to the high plasmid copy number.
Encodes a chimera in which aa 1-48 of processed human factor VII (Gla domain), with mutations K32E, K38T, L39E, replace the corresponding region of factor IX.
GenBank Number J02933
Vector Construct size (kb) 7.400000095367432
DESCRIPTION OF VECTOR COMPONENT Name of vector pCMV5Intact vector size 4.700 Type of vector phagemid Vector end BamHI Vector end BamHI Cloning sites EcoRI BamHI HindIII Polylinker sites Host range mammalian cells; Escherichia coliFeatures (with orientation and position when available) promoter CMV, -> terminator hGH, -> replicon SV40 replicon pMB1 marker(s) ampR, replicon f1, Cross references
Insert DNA cDNA
DESCRIPTION OF INSERT COMPONENT Genome Homo sapiens Gene symbol F7Genomic copy number unique Gene name coagulation factor VII (serum prothrombin conversion accelerator)Contains complete coding sequence? U Chromosome 13; Localization 13 q34 Genome Homo sapiens Gene symbol F9Genomic copy number unique Gene name coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B)Contains complete coding sequence? U Chromosome X; Localization X q26.3-q27.1 Type of DNA cDNA Insert end BamHI Insert end BamHI Insert size (kb) 2.7 Cross references DNA Seq. Acc. J02933
Insert lengths(kb) 2.700000047683716
Gene product coagulation factor VII (serum prothrombin conversion accelerator) [F7]
Alleles A1, A2, D2, C1, D1, C2
Target Gene coagulation factor VII (serum prothrombin conversion accelerator)
Insert Size (kb) 2.700
Shipping Information Distributed freeze-dried