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ATCC701068 Y145 [135]
基本信息
资源编号 ATCC701068
资源名称 Y145 [135]
种属 Rattus norvegicus, rat
安全等级 1
培养方法
培养基 ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
详细说明
GenBank Number R46917
Vector DESCRIPTION OF VECTOR COMPONENT Name of vector pBluescript SK-Intact vector size 2.960 Type of vector phagemid Vector end EcoRI Vector end XhoI Cloning sites KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Polylinker sites SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI (Eco0109I DraII ApaI) KpnI Construction pUC19 Host range Escherichia coliFeatures (with orientation and position when available) replicon f1, -> insert detection lacZ', primer site M13-20, -> promoter T7, -> MCS KpnI...SacI, -> promoter T3, promoter lac, primer site M13 reverse, replicon pMB1 marker(s) ampR, Cross references
Insert DNA cDNA
DESCRIPTION OF INSERT COMPONENT Genome rat Gene name DNA Segment, expressedContains complete coding sequence? U Strain Sprague-Dawley Tissue incisor, maxillary and mandibular (apical portion and entire pulp) from animals 3-4 weeks old Type of DNA cDNA Insert 5' end EcoRI Modification EcoRI adaptor Insert 3' end XhoI Modification XhoI primer Insert size (kb) Cross references DNA Seq. Acc. R46917
Tissue incisor, maxillary and mandibular (apical portion and entire pulp) from animals 3-4 weeks old
Gene product DNA Segment, expressed
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