uedbet新版 > 美国ATCC > 克隆载体和分子克隆
价格:请临时咨询
数量:
ATCC81471D HFBCP52
基本信息
资源编号 ATCC81471D
资源名称 HFBCP52
种属 Homo sapiens, human
安全等级 1
培养方法
详细说明
描述 Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.4; PstI--3.3, 1.05; HindIII--4.4.
EST02418 matches EMBL accession MMF52 with 92.43% identity over the sequence range: 1-238.
This sequence record is for mouse myristoylated alanine-rich protein kinase C substrate (Macs).
GenBank Number M85894
Vector Construct size (kb) 4.400000095367432
DESCRIPTION OF VECTOR COMPONENT Name of vector pBluescript SK-Intact vector size 2.960 Type of vector phagemid Vector end EcoRI Vector end EcoRI Cloning sites KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Polylinker sites SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI (Eco0109I DraII ApaI) KpnI Construction pUC19 Host range Escherichia coliFeatures (with orientation and position when available) replicon f1, -> insert detection lacZ', primer site M13-20, -> promoter T7, -> MCS KpnI...SacI, -> promoter T3, promoter lac, primer site M13 reverse, replicon pMB1 marker(s) ampR, Cross references
Insert DNA cDNA
DESCRIPTION OF INSERT COMPONENT Genome Homo sapiens Gene symbol D0S582EGenomic copy number unique Gene name DNA Segment, single copy, expressedContains complete coding sequence? U Sequence homology Homo sapiens myristoylated alanine-rich protein kinase C substrate Tissue fetal brain, female, 17-18 wk (Stratagene 936206) Type of DNA cDNA Insert end Modification EcoRI linkers Insert end Modification EcoRI linkers Insert size (kb) 1.4 Cross references DNA Seq. Acc. M85894; EST EST02418; Primer M13 Forward; THC id THC123978
Insert lengths(kb) 1.399999976158142
Tissue fetal brain, female, 17-18 wk (Stratagene 936206)
Gene product myristoylated alanine-rich protein kinase C substrate(myristoylated alanine-rich protein kinase C substrate (MARCKS)) [Macs]
Insert Size (kb) 1.400
Shipping Information Distributed DNA (dried). Rehydrate with TE. (amount 200 ng)