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ATCCHB-201 AF4-73.3.1
基本信息
资源编号 ATCCHB-201
资源名称 AF4-73.3.1
种属 Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
类型 hybridoma: B lymphocyte
形态 lymphoblast
提供形式 frozen
安全等级 1
应用领域 The antibody reacts with cells bearing the e and o haplotypes at the Igh-C locus.
Tested and found negative for ectromelia virus (mousepox).
培养方法
培养基 Culture Medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 1 mM Na pyruvate, L-glutamine, 2 mM, 90%; fetal bovine serum, 10%.
传代方法 Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp4 cells/ml and maintain between 5 X 10 exp4 and 5 X 10 exp5 cells/ml.
生长特性 suspension
详细说明
描述 Animals were immunized with spleen cells from A/J mice.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody reacts with cells bearing the e and o haplotypes at the Igh-C locus.
Tested and found negative for ectromelia virus (mousepox).
Derivation Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed immunoglobulin; monoclonal antibody; against Igh-5.5 (IgD e)
Cellular Products immunoglobulin; monoclonal antibody; against Igh-5.5 (IgD e)
Isotype IgG2b